Anti-HLA-A1:MAGE-A1 complex [HYB3]

Absolute Antibody

Anti-HLA-A1:MAGE-A1 complex [HYB3]

9

This antibody is specific for human HLA-A1:MAGE-A1 complex.

The structure of the fab version of this antibody in complex with HLA-A1 and MAGE-A1 was eludicated using X-ray crystallography. This was done to determine how the antibody binds to its ligand (Huelsmeyer et al, 2005; pmid:15537658). To characterize this antibody flow cytometry was preformed. Flow cytometry was preformed with the fab version of this antibody on dendritic cells (Chames et al, 2002; pmid:12097420). To assess the expression of low and high affinity scFv-y CD28 and scFv- receptors on gene-transduced primary human T lymphocytes. Flow cytometry was preformed on gene-transduced primary human T lymphocytes using the scFv version of this antibody. Furthermore a cytotoxic assay was preformed on scFv-y + CD28 or scFv-transduced human T lymphocytes. The cytotoxic assay was preformed using Cr51 release assay (Willemsen et al, 2005; pmid:15944290). To characterize this antibody after expressing the antibody in E.coli. An ELISA was preformed using the fab version of this antibody on a biotinylated M1/A1 complex. Furthermore to asses the ability of this antibody to bind M1/A1-positive cells. Flow cytometry was preformed on these cells using the scFv version of this antibody (Saeed et al, 2016; pmid:27022262).

Functional Assay, Flow Cytometry, ELISA

Research Use Only

Monoclonal

HYB3

Human

IgG1

This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203