Anti-HLA-DR [L243]

Absolute Antibody

Anti-HLA-DR [L243]

7

This antibody reacts with the HLA-DR antigen and does not cross react with HLA-DP or HLA-DQ. This antibody binds a conformational epitope on HLA-DRa which depends on the correct folding of the abeta heterodimer. HLA-DR is an MHC class II cell surface rece

This antibody can be used for the immunoprecipitation of HLA-DR and MHC class-II peptides (PMID: 2961843; 11390619). L243 selectively inhibited the in vitro proliferation of Epstein-Barr virus-transformed lymphoblastoid cell lines (LCL) (PMID: 2467753). This antibody was used in a flow cytometric assay to detect the decrease in HLA-DR expression on monocytes which is an indicator of immunosuppression in patients with sepsis and is correlated with increased risk of secondary infection and mortality (PMID: 33432735). Alexa 568 and FITC labelled L243 antibody was used in the immunofluorescent staining of renal microvascular endothelial cells (RMEC) or human kidney tissue (PMID: 12707403). This antibody was also used in the detection of MHC class II protein in a western blot (PMID: 11390619). This antibody can also be used for the immunocytochemical and immunohistochemical analysis of frozen sections of necropsy brain tissue to identify HLA-DR expressing cells (PMID: 1864982).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking

FC; IF; IP; WB; ICC; IHC; block

Research Use Only

Monoclonal

L243

CD74

CD74 molecule

CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DHLAG; gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia-associated invariant chain; Ia-GAMMA; II; MHC HLA-DR gamma chain; p33

Rabbit

IgG

HLA class II histocompatibility antigen gamma chain

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing mice with the human B cell line RPMI 8866.

-20°C,2°C to 8°C

12352203