Anti-HLA-DR [L243]

Absolute Antibody

Anti-HLA-DR [L243]

9

This antibody can be used for the immunoprecipitation of HLA-DR and MHC class-II peptides (Bonnefoy et al., 1988; PMID: 2961843). This antibody selectively inhibited the in vitro proliferation of Epstein-Barr virus-transformed lymphoblastoid cell lines (Kabelitz and Janssen, 1989; PMID: 2467753). This antibody can also be used for the immunocytochemical and immunohistochemical analysis of frozen sections of necropsy brain tissue to identify HLA-DR-expressing cells (Esiri et al., 1991; PMID: 1864982). This antibody was used in the detection of MHC class II protein in a western blot (Esser et al., 2001; PMID: 11390619). This antibody was used in a flow cytometric assay to detect the decrease in HLA-DR expression on monocytes, which is an indicator of immunosuppression in patients with sepsis and is correlated with an increased risk of secondary infection and mortality (Quadrini et al., 2021; PMID: 33432735). Alexa 568- and FITC-labeled versions of this antibody were used in the immunofluorescent staining of renal microvascular endothelial cells (RMEC) or human kidney tissue (Muczynski et al., 2003; PMID: 12707403). This antibody was assessed in a pilot study using a canine lymphoma model. In vitro studies showed that this antibody binds to both healthy and lymphoma canine lymphocytes, inducing apoptosis in canine non-Hodgkin lymphoma cells. In vivo studies confirmed the antibodys safety in healthy and lymphoma-affected dogs, as well as its binding activity in lymphoma-affected lymph nodes. Preliminary results indicated transient disease stabilization in a subset of dogs with advanced lymphoma (Stein et al., 2011; PMID: 21133722).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

L243

CD74

CD74 molecule

CLIP, DHLAG, HLADG, II, Ia-GAMMA, p33, HLA class II histocompatibility antigen gamma chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 molecule, major histocompatibility complex, class II invariant chain, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma chain, class II MHC-associated invariant chain peptide, gamma chain of class II antigens

Rabbit

IgG

HLA class II histocompatibility antigen gamma chain

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

41116161