Anti-HLA-DR10 [Lym-1]

Absolute Antibody

Anti-HLA-DR10 [Lym-1]

9

This antibody could recognize cell lines of B-cell lineage but not cells of T-cell, myeloid, or erythroid derivation in a live cell radioimmunoassay. Flow cytometric analysis of 22 lymphoma biopsies showed that the majority of B-cell tumors were LYM-1 positive. Approximately 40% of B-cell chronic lymphocytic leukemias were found to be positive with Lym-1. The binding of this antibody to peripheral blot lymphocytes was assessed using indirect immunofluorescence staining. Immunoperoxidase staining of nonlymphoid human organs and tissues revealed weak reactivity of Lym-1. This antibody was also capable of immunoprecipitating HLA-DR10 antigen from Raji cells and ARH-77 myeloma cells. (PMID: 3542194). This antibody also was used for immunohistochemical staining of advanced B-cell NHL tissues from patients (PMID: 10809213). This antibody can be used to identify the HLA-DR variant HLA-DR10 in a western blot (PMID: 8917632). LYM-1 chimeric antigen receptor T cells were demonstrated to exhibit potent anti-tumor effects against B-cell lymphoma (PMID: 29261129).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, RadioImmunoAssay

Research Use Only

Monoclonal

Lym-1

CD74

CD74 molecule

CLIP, DHLAG, HLADG, II, Ia-GAMMA, p33, HLA class II histocompatibility antigen gamma chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 molecule, major histocompatibility complex, class II invariant chain, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma chain, class II MHC-associated invariant chain peptide, gamma chain of class II antigens

Human

IgG1

HLA class II histocompatibility antigen gamma chain

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203