Anti-HMGB1 [2G7], Mouse IgG2b, Fc Silent(tm), kappa

Absolute Antibody

Anti-HMGB1 [2G7], Mouse IgG2b, Fc Silent(tm), kappa

9

This antibody is specific for amino acids 53-63 of HMGB1.

The original antibody was used for an ELISA on recombinant rat HMGB1. This antibody was used for a western blot on recombinant rat HMGB1. The epitope of this antibody was determined using peptide binding. Mice were subjected to a cecal ligation and puncture, after which this antibody was administered. It showed an increased survival rate in these septic mice (US7632500B2). This antibody was used for immunofluorescence on RAW 264.7 cells, to study intracellular HMGB1. This antibody was used for an ELISPOT on RAW 264.7, C57 and HMC-12 cells (Waehaemaa et al., 2007; PMID:16980509). This antibody was administered to mice subjected to ACLT, the antibody was able to protect cartilage (Aulin et al., 2020; PMID:31982563). This antibody was tested in two murine arthritis models, it showed a decrease in general disease severity (Schierbeck et al., 2011; PMID:21666956). This antibody was used in vivo on SOD1G93A transgenic mice, to test the effect of this antibody on ALS, the antibody did not increase survival time but it did improve early motor deficits and reduced inflammation (Lee et al., 2019; PMID:30782181).

ImmunoFluorescence, Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

2G7

Hmgb1

high mobility group box 1

Ac2-008; Ac2-008-like; amphoterin; heparin-binding protein p30; high mobility group 1; high mobility group protein 1; high mobility group protein B1; Hmg1; HMG-1

Mouse

IgG2b

High mobility group protein B1

This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

Rat

-20°C,2°C to 8°C

12352203