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Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

Anti-HMGB2 Antibody

HPA003506
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHMGB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HMGB2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3148
  • Target name
    HMGB2
  • Target description
    high mobility group box 2
  • Target synonyms
    HMG2, high mobility group protein B2, HMG-2, high mobility group protein 2, high-mobility group (nonhistone chromosomal) protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26583
  • Protein Name
    High mobility group protein B2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HMGB2 using anti-HMGB2 antibody (PB10002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB2 antigen affinity purified polyclonal antibody (Catalog # PB10002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGB2 at approximately 24 kDa. The expected band size for HMGB2 is at 24 kDa.
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