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Immunohistochemical staining of human gastrointestinal shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gastrointestinal shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gastrointestinal shows moderate granular cytoplasmic positivity in glandular cells.

Anti-HMGCR Antibody

HPA008338
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetHMGCR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HMGCR
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3156
  • Target name
    HMGCR
  • Target description
    3-hydroxy-3-methylglutaryl-CoA reductase
  • Target synonyms
    3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-Coenzyme A reductase; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; LDLCQ3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04035
  • Protein Name
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HMGCR using anti-HMGCR antibody (A00643-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGCR antigen affinity purified polyclonal antibody (Catalog # A00643-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGCR at approximately 97 kDa. The expected band size for HMGCR is at 97 kDa.
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