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anti-hnRNP U antibody

ARG54077
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
TargetHNRNPU
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-hnRNP U antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3192
  • Target name
    HNRNPU
  • Target description
    heterogeneous nuclear ribonucleoprotein U
  • Target synonyms
    DEE54, EIEE54, GRIP120, HNRNPU-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120, heterogeneous nuclear ribonucleoprotein U, HNRNPU antisense RNA 1, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), nuclear p120 ribonucleoprotein, p120 nuclear protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP U/p120/HNRNPU antigen affinity purified polyclonal antibody (Catalog # A03691-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP U/p120/HNRNPU at approximately 120 kDa. The expected band size for hnRNP U/p120/HNRNPU is at 90 kDa.
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