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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-HOOK3 Antibody

HPA024756
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHOOK3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HOOK3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84376
  • Target name
    HOOK3
  • Target description
    hook microtubule tethering protein 3
  • Target synonyms
    hHK3; h-hook3; HK3; hook homolog 3; protein Hook homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86VS8
  • Protein Name
    Protein Hook homolog 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: HOOK3 antibody at 4.9microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa
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Figure 1. Western blot analysis of HOOK3 using anti-HOOK3 antibody (A07701-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOOK3 antigen affinity purified polyclonal antibody (Catalog # A07701-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOOK3 at approximately 83 kDa. The expected band size for HOOK3 is at 83 kDa.
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