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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.

Anti-HOXC4 Antibody

HPA053910
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHOXC4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HOXC4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3221
  • Target name
    HOXC4
  • Target description
    homeobox C4
  • Target synonyms
    cp19; homeo box 3E; homeobox protein CP19; homeobox protein Hox-3E; homeobox protein Hox-C4; HOX3; HOX3E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09017
  • Protein Name
    Homeobox protein Hox-C4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC4 antigen affinity purified polyclonal antibody (Catalog # A09869-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC4 at approximately 36 kDa. The expected band size for HOXC4 is at 36 kDa.
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