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Anti-HPV16-L1 [H16.V5 ]

Research Use Only
AB02722-3.0-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityVirus
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-HPV16-L1 [H16.V5 ]
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody recognizes a surface confirmation epitope on the intact HPV-16 L1 virus like particles. This antibody is known to recognize the FG and HI loops, which are immunodominant in the humoral response against the HPV16 major capsid protein.
  • Application Supplier Note
    The specificity of this antibody against intact and disrupted virus-like particles (VLPs) of HPV-6, HPV-16, HPV-11, and HPV-18 was determined using ELISA (PMID: 8874508). This antibody was used as a capture antibody to test human serum IgG reactive with HPV-16 capsids (PMID: 8896492). This antibody was also reported to block HPV-16 VLP-induced agglutination of mouse RBC (PMID: 8806551). This antibody is capable of neutralizing by inhibiting HPV-16 binding to cell surfaces (PMID: 9223527). This antibody was also used to characterize a major neutralizing epitope on the HPV-16 type virus (PMID: 10233949). Sequences at both ends of the long FG loop (amino acids 260 to 290) were required for H16.V5 reactivity (PMID: 14557648). H16.V5 is often used for the assessment of the integrity and antigenicity of VLPs in vaccine products (PMID: 17012876). This antibody, when conjugated with magnetic beads, can also be used to immunoprecipitate HPV16 VLP and PsV out of cell lysates (PMID: 22927980). The binding to the surfaces of CaSki cells was detected by indirect immunofluorescence staining with H16.V5 and flow cytometry. The ability of gradient-purified HPV16 L1 to bind to the surface of SiHa cells at 4°C was detected by flow cytometric staining with the H16.V5 monoclonal antibody (PMID: 15890912).
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    H16.V5
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP03101
  • Protein Name
    Major capsid protein L1
  • Reactivity
    Virus
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203