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Immunohistochemical staining of human liver shows moderate positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate positivity in hepatocytes.

Anti-HSD17B7 Antibody

HPA047496
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSD17B7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSD17B7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51478
  • Target name
    HSD17B7
  • Target description
    hydroxysteroid 17-beta dehydrogenase 7
  • Target synonyms
    PRAP, SDR37C1, 3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7, 17 beta-hydroxysteroid dehydrogenase type VII, 17-beta-HSD 7, 17-beta-hydroxysteroid dehydrogenase 7, 17beta hydroxysteroid dehydrogenase, 3-keto-steroid reductase, dihydrotestosterone oxidoreductase, estradiol 17-beta-dehydrogenase 7, short chain dehydrogenase/reductase family 37C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56937
  • Protein Name
    3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B7 antigen affinity purified polyclonal antibody (Catalog # A05850-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B7 at approximately 38 kDa. The expected band size for HSD17B7 is at 38 kDa.
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