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Immunohistochemical staining of human testis shows weak to moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows weak to moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows weak to moderate nuclear positivity in cells in seminiferous ducts.

Anti-HSF2 Antibody

HPA031455
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSF2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3298
  • Target name
    HSF2
  • Target description
    heat shock transcription factor 2
  • Target synonyms
    HSF 2, HSTF 2, heat shock factor protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03933
  • Protein Name
    Heat shock factor protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
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