Figure 1. IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). Hsp90 Alpha was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). Hsp90 Alpha was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband(r)
PB9089-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetHSP90AA1
Overview
- SupplierBoster Bio
- Product NameAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteWB: The detection limit for Hsp90 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID3320
- Target nameHSP90AA1
- Target descriptionheat shock protein 90 alpha family class A member 1
- Target synonymsEL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2, heat shock protein HSP 90-alpha, HSP 86, LPS-associated protein 2, epididymis luminal secretory protein 52, epididymis secretory sperm binding protein Li 65p, heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein 90kDa alpha family class A member 1, heat shock protein family C member 1, lipopolysaccharide-associated protein 2, renal carcinoma antigen NY-REN-38
- HostRabbit
- IsotypeIgG
- Protein IDP07900
- Protein NameHeat shock protein HSP 90-alpha
- Scientific DescriptionBoster Bio Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband® catalog # PB9089. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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