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Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using HPA008424 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using HPA008424 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using HPA008424 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.

Anti-HSP90B1-25ul

HPA008424
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSP90B1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHC, ICC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14625
  • Protein Name
    Endoplasmin
  • Scientific Description
    Rabbit Polyclonal Anti-HSP90B1 Antibody against Human heat shock protein 90kDa beta (Grp94), member 1. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human, Mouse
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRP94 using anti-GRP94 antibody (PB9637). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: huamn CACO-2 whole cell lysates, Lane 5: huamn THP-1 whole cell lysates, Lane 6: huamn CACO-2 whole cell lysates, Lane 7: huamn HepG2 whole cell lysates, Lane 8: huamn HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94 antigen affinity purified polyclonal antibody (Catalog # PB9637) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94 at approximately 100 kDa. The expected band size for GRP94 is at 92 kDa.
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