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Immunohistochemical staining of human duodenum shows strong cytoplasm granular positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasm granular positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasm granular positivity in glandular cells.

Anti-HTRA2 Antibody

HPA027366
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHTRA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HTRA2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27429
  • Target name
    HTRA2
  • Target description
    HtrA serine peptidase 2
  • Target synonyms
    MGCA8, OMI, PARK13, PRSS25, serine protease HTRA2, mitochondrial, HtrA-like serine protease, Omi stress-regulated endoprotease, epididymis secretory sperm binding protein, high temperature requirement protein A2, protease, serine, 25, serine protease 25, serine proteinase OMI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43464
  • Protein Name
    Serine protease HTRA2, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HTRA2 using anti-HTRA2 antibody (A01941-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HT-1080 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA2 antigen affinity purified polyclonal antibody (Catalog # A01941-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA2 at approximately 36 kDa. The expected band size for HTRA2 is at 36,49 kDa.
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