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Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Anti-HTRA4 Antibody

HPA045402
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHTRA4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HTRA4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID203100
  • Target name
    HTRA4
  • Target description
    HtrA serine peptidase 4
  • Target synonyms
    high-temperature requirement factor A4; probable serine protease HTRA4; serine protease HTRA4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP83105
  • Protein Name
    Serine protease HTRA4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HTRA4 using anti-HTRA4 antibody (A13079-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA4 antigen affinity purified polyclonal antibody (Catalog # A13079-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA4 at approximately 51 kDa. The expected band size for HTRA4 is at 51 kDa.
Product group Antibodies
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Anti-HTRA4 Antibody Picoband(r)A13079-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetHTRA4
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Product group Antibodies
Cusabio
HTRA4 AntibodyCSB-PA010904GA01HU
ApplicationsWestern Blot, ELISA
TargetHTRA4
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Product group Antibodies
Biosynth
HTRA4 antibody70R-17857
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Atlas Antibodies
PrEST Antigen HTRA4APREST76369
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DU145 lysates probed with HTRA4 Polyclonal Antibody, Unconjugated (bs-18101R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
HTRA4 Polyclonal AntibodyBS-18101R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetHTRA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HTRA4 AntibodyLS-C830160
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetHTRA4
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