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ProductsBack/Anti-Hu CD56 Biotin

Anti-Hu CD56 Biotin

1B-789
EXBIO
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-Hu CD56 Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Flow cytometry: Recommended dilution: 1-5 microg/ml. Western blotting: Non-reducing conditions.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clone ID
    LT56
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Isotype
    IgG2a
  • Scientific Description
    CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (Catalog # A00184-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 150KD. The expected band size for NCAM1 is at 150KD.
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