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Anti-human CCL2 mAb (MTBH5), unconjugated

Anti-human CCL2 mAb (MTBH5), unconjugated

3491-3
Mabtech
ApplicationsELISA
Product group Antibodies
TargetCCL2
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human CCL2 mAb (MTBH5), unconjugated
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MTBH5
  • Conjugate
    Unconjugated
  • Gene ID6347
  • Target name
    CCL2
  • Target description
    C-C motif chemokine ligand 2
  • Target synonyms
    C-C motif chemokine 2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP1; MCP-1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; SCYA2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; SMC-CF
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enable specific detection of human CCL2 (MCP-1) in immunoassays such as ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCP-1 using anti-MCP-1 antibody (PB9570). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP-1 antigen affinity purified polyclonal antibody (Catalog # PB9570) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP-1 at approximately 11 kDa. The expected band size for MCP-1 is at 11 kDa.
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