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Anti-Human CD133 Antibody, Purified

136-00095
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetProm1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Human CD133 Antibody, Purified
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HB#7
  • Gene ID19126
  • Target name
    Prom1
  • Target description
    prominin 1
  • Target synonyms
    4932416E19Rik, AC133, CD133, Prom, Prom-1, Proml1, prominin-1, antigen AC133 homolog, prominin-like 1, prominin-like protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO54990
  • Protein Name
    Prominin-1
  • Scientific Description
    Human CD133 Antibody [clone: HB#7], monoclonal Mouse IgG1. Validated for flow cytometry.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD133/Prom1 using anti-CD133/Prom1 antibody (A01767-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates, Lane 2: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD133/Prom1 antigen affinity purified polyclonal antibody (Catalog # A01767-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD133/Prom1 at approximately 130 kDa. The expected band size for CD133/Prom1 is at 130 kDa.
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