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Anti-human EBI3 mAb (MT140), biotin

Anti-human EBI3 mAb (MT140), biotin

3458-6
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
TargetIL27
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human EBI3 mAb (MT140), biotin
  • Delivery Days Customer
    5
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT140
  • Conjugate
    Biotin
  • Gene ID246778
  • Target name
    IL27
  • Target description
    interleukin 27
  • Target synonyms
    IL-27, IL-27A, IL27A, IL27p28, IL30, p28, interleukin-27 subunit alpha, IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27-A, interleukin-30
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human IL-27 and the EBI3 subunit of IL-27 and IL-35 in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IL27 using anti-IL27 antibody (A00857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: recombinant human IL27 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL27 antigen affinity purified polyclonal antibody (Catalog # A00857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL27 at approximately 60KD. The expected band size for IL27 is at 50KD.
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