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Anti-human IL-3 mAb (IL3B), biotin

Anti-human IL-3 mAb (IL3B), biotin

3435-6
Mabtech
ApplicationsELISA, ELISpot Assay
DownloadMSDS
Product group Antibodies
TargetIL3
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human IL-3 mAb (IL3B), biotin
  • Delivery Days Customer
    5
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    IL3B
  • Conjugate
    Biotin
  • Gene ID3562
  • Target name
    IL3
  • Target description
    interleukin 3
  • Target synonyms
    colony-stimulating factor, multiple; hematopoietic growth factor; IL-3; interleukin-3; mast-cell growth factor; MCGF; MULTI-CSF; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; P-cell stimulating factor
  • Host
    Rat
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human IL-3 in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

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Figure. Western blot analysis of IL-3 using anti-IL-3 antibody (RP1008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-3 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-3 antigen affinity purified polyclonal antibody (Catalog # RP1008) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-3 at approximately 15KD. The expected band size for IL-3 is at 15KD.
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