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Anti-human IP-10 mAb (MTP13), biotin

Anti-human IP-10 mAb (MTP13), biotin

3401-6
Mabtech
ApplicationsELISpot Assay
Product group Antibodies
TargetCXCL10
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human IP-10 mAb (MTP13), biotin
  • Delivery Days Customer
    9
  • Applications
    ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MTP13
  • Conjugate
    Biotin
  • Gene ID3627
  • Target name
    CXCL10
  • Target description
    C-X-C motif chemokine ligand 10
  • Target synonyms
    C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, gamma IP10, interferon-inducible cytokine IP-10, protein 10 from interferon (gamma)-induced cell line, small inducible cytokine subfamily B (Cys-X-Cys), member 10, small-inducible cytokine B10
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human IP-10 in ELISpot.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IP10 using anti-IP10 antibody (PB9695). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP10 antigen affinity purified polyclonal antibody (Catalog # PB9695) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP10 at approximately 34 kDa. The expected band size for IP10 is at 11 kDa.
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