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Immunohistochemical staining of human ovary shows cytoplasmic positivity in ovarian stroma cells.
Immunohistochemical staining of human ovary shows cytoplasmic positivity in ovarian stroma cells.
Immunohistochemical staining of human ovary shows cytoplasmic positivity in ovarian stroma cells.

Anti-HYI Antibody

HPA040092
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHYI
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HYI
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81888
  • Target name
    HYI
  • Target description
    hydroxypyruvate isomerase (putative)
  • Target synonyms
    endothelial cell apoptosis protein E-CE1; HT036; hydroxypyruvate isomerase homolog; putative hydroxypyruvate isomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T013
  • Protein Name
    Putative hydroxypyruvate isomerase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA719395LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of HYI using anti-HYI antibody (A14191-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HYI antigen affinity purified polyclonal antibody (Catalog # A14191-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HYI at approximately 30KD. The expected band size for HYI is at 30KD.
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