Anti-IDH1 mutant [MsMab-1]

Absolute Antibody

Anti-IDH1 mutant [MsMab-1]

9

The binding reactivity of this antibody to wild-type IDH1 and its mutants (R132H, R132C, R132S, R132G and R132L) peptides was determined using ELISA. Western blot analysis also revealed that MsMab-1 reacted with recombinant proteins of IDH1-R132H, IDH1-R132S, and IDH1-R132G, but not with wild type IDH1 and other IDH1 mutations. This antibody also recognizes IDH2-R172M protein, despite that the IDH1-R132G peptide shows only 73.7% identity with the equivalent portion of IDH2-R172M (GGTKPITIGMHAHGDQYKA). This antibody successfully stained the IDH1-R132S or IDH1-R132G-expressing glioma cells in immunohistochemistry (PMID: 23782684). This antibody can bind IDH2-R172S mutant of IDH2 in an ELISA and can recognize PA-tagged IDH2-R172S recombinant protein in a western blot. This antibody was also used to stain IDH2-R172S-expressing osteosarcoma tissues in immunohistochemistry (PMID: 24403254). A complete summary of the reactivity of this antibody towards various IDH1 and IDH2 mutants can be found in Liu et. al, 2013 (PMID: 24403254).

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

MsMab-1

IDH1

isocitrate dehydrogenase (NADP(+)) 1

HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD, isocitrate dehydrogenase [NADP] cytoplasmic, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, epididymis luminal protein 216, epididymis secretory protein Li 26, epididymis secretory sperm binding protein, isocitrate dehydrogenase (NADP(+)) 1, cytosolic, isocitrate dehydrogenase 1 (NADP+), soluble, oxalosuccinate decarboxylase

Mouse

IgG1

Isocitrate dehydrogenase [NADP] cytoplasmic

-20°C,2°C to 8°C

12352203