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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Anti-IDH3G Antibody

HPA002017
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIDH3G
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IDH3G
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3421
  • Target name
    IDH3G
  • Target description
    isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma
  • Target synonyms
    H-IDHG, RP99, isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, IDH-gamma, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD(+)-specific ICDH subunit gamma, NAD+-specific ICDH, isocitrate dehydrogenase (NAD(+)) 3 gamma, isocitrate dehydrogenase 3 (NAD(+)) gamma, isocitrate dehydrogenase 3 (NAD+) gamma, isocitrate dehydrogenase 3 gamma, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit, isocitric dehydrogenase subunit gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51553
  • Protein Name
    Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of IDH3G using anti-IDH3G antibody (A10370-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3G antigen affinity purified polyclonal antibody (Catalog # A10370-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH3G at approximately 40 kDa. The expected band size for IDH3G is at 43 kDa.
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