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Anti-IDNK (N-term) Antibody

102-24948
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIDNK
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IDNK (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB54309
  • Gene ID414328
  • Target name
    IDNK
  • Target description
    IDNK gluconokinase
  • Target synonyms
    C9orf103, bA522I20.2, hGntK, probable gluconokinase, putative gluconokinase, glucokinase-like protein, gluconate kinase, gluconokinase-like protein, idnK, gluconokinase homolog
  • Host
    Rabbit
  • Protein IDQ5T6J7
  • Protein Name
    Probable gluconokinase
  • Scientific Description
    Rabbit Anti-IDNK (N-Term) Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDNK using anti-IDNK antibody (A15308-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat kisney tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDNK antigen affinity purified polyclonal antibody (Catalog # A15308-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDNK at approximately 21 kDa. The expected band size for IDNK is at 21 kDa.
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