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Anti-IDUA Antibody

144-66792
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIDUA
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IDUA Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID3425
  • Target name
    IDUA
  • Target description
    alpha-L-iduronidase
  • Target synonyms
    IDA, MPS1, MPSI, alpha-L-iduronidase, iduronidase alpha-L-, mucopolysaccharidosis type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35475
  • Protein Name
    Alpha-L-iduronidase
  • Scientific Description
    IDUA pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDUA using anti-IDUA antibody (A02281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDUA antigen affinity purified polyclonal antibody (Catalog # A02281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDUA at approximately 73 kDa. The expected band size for IDUA is at 73 kDa.
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