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Immunohistochemical staining of human Endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Endometrium shows moderate cytoplasmic positivity in glandular cells.

Anti-IDUA Antibody

HPA046979
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIDUA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IDUA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3425
  • Target name
    IDUA
  • Target description
    alpha-L-iduronidase
  • Target synonyms
    alpha-L-iduronidase; IDA; iduronidase alpha-L-; MPS1; MPSI; mucopolysaccharidosis type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35475
  • Protein Name
    Alpha-L-iduronidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA011000LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of IDUA using anti-IDUA antibody (A02281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDUA antigen affinity purified polyclonal antibody (Catalog # A02281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDUA at approximately 73 kDa. The expected band size for IDUA is at 73 kDa.
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