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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-IFFO1 Antibody

HPA069344
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIFFO1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IFFO1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25900
  • Target name
    IFFO1
  • Target description
    intermediate filament family orphan 1
  • Target synonyms
    HOM-TES-103, IFFO, non-homologous end joining factor IFFO1, NHEJ factor IFFO1, intermediate filament-like MGC:2625
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ0D2I5
  • Protein Name
    Non-homologous end joining factor IFFO1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of IFFO1 using anti-IFFO1 antibody (A17273-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFFO1 antigen affinity purified polyclonal antibody (Catalog # A17273-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFFO1 at approximately 30 kDa. The expected band size for IFFO1 is at 62 kDa.
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