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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.

Anti-IFIT5 Antibody

HPA037957
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIFIT5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IFIT5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24138
  • Target name
    IFIT5
  • Target description
    interferon induced protein with tetratricopeptide repeats 5
  • Target synonyms
    ISG58, P58, RI58, interferon-induced protein with tetratricopeptide repeats 5, IFIT-5, interferon-induced 58 kDa protein, retinoic acid- and interferon-inducible 58 kDa protein, retinoic acid- and interferon-inducible protein (58kD)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13325
  • Protein Name
    Interferon-induced protein with tetratricopeptide repeats 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of IFIT5 using anti-IFIT5 antibody (A07415-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFIT5 antigen affinity purified polyclonal antibody (Catalog # A07415-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFIT5 at approximately 56 kDa. The expected band size for IFIT5 is at 56 kDa.
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