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Anti-IFN gamma [AF2]

AB00725-3.0-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetIFNG
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IFN gamma [AF2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody binds specifically to IFNgamma, a dimerised soluble cytokine member of the type II class of interferons synthesised by activated T-lymphocytes and natural killer (NK) cells. . IFNgamma plays a role in both the innate and adaptive immune responses, an
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AF2
  • Gene ID3458
  • Target name
    IFNG
  • Target description
    interferon gamma
  • Target synonyms
    IFG; IFI; IFN-gamma; IMD69; immune interferon; interferon gamma
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP01579
  • Protein Name
    Interferon gamma
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19 kDa. The expected band size for IFNG is at 19 kDa.
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