Anti-IFNAR-1 [64G12]

Absolute Antibody

Anti-IFNAR-1 [64G12]

7

This antibody is capable of nuetralizing the biologic activity of IFN-alpha, -beta, and -omega and natural type I IFN, but not IFN-gamma. The receptor-blocking anti-IFNAR-1 mAb 64G12 also inhibited IFN-a2a-induced ISGF3 and IFN regulatory factor-1 (IRF-1) binding to their ISRE. It is also reported that the affinity of this antibody for the cellular receptor depends on the cell line used. The binding characterization of this antibody was done using ELISA and western blotting (PMID: 8423335). The reactivity of the monoclonal antibodies (mAbs) recognizing the recombinant soluble IFN-R was tested against the natural class I receptor expressed at the surface of Daudi cells, by membrane immunofluorescence. Antibody 64G12 both inhibits the binding of radiolabeled interferon-alpha2 and IFN-alpha8 to their cell surface receptors and neutralizes the antiviral and antiproliferative actions of all the type I interferons tested, including IFN-beta, IFN-omega, and human leukocyte IFN, a mixture of different interferon-alpha isotypes. Immunoprecipitation followed by surface iodination demonstrated that the neutralizing MAb 64G12 recognizes a protein of 105 kD in extracts of Daudi cells (PMID: 7584665). This antibody inhibits activation of the Jak-Stat signal transduction pathway, administered together with a subeffective dose of cyclosporine induced prolonged survival of skin allografts in major histocompatibility complex (MHC) divergent cynomolgus monkeys (PMID: 9568730). This antibody was also used to study the expression of IFN-alpha/beta receptor expression on a number of different human cell lines including Daudi, K562, Ly28 and HL60 using confocal microscopy and flow cytometry (PMID: 12040445).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking, Other Application

ELISA; WB; IF; Inhibit; NTRL; IP; FC; confocal microscopy

Research Use Only

Interferon alpha/beta receptor 1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203