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Anti-IFNB1 Antibody

102-20237
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIFNB1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IFNB1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1394CT509.46.3
  • Gene ID3456
  • Target name
    IFNB1
  • Target description
    interferon beta 1
  • Target synonyms
    fibroblast interferon; IFB; IFF; IFNB; IFN-beta; interferon beta; interferon, beta 1, fibroblast; interferon-beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01574
  • Protein Name
    Interferon beta
  • Scientific Description
    Mouse Anti-IFNB1 Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 4/ITGA4 using anti-Integrin Alpha 4/ITGA4 antibody (A00468-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 4/ITGA4 antigen affinity purified polyclonal antibody (Catalog # A00468-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 4/ITGA4 at approximately 140-150 kDa. The expected band size for Integrin Alpha 4/ITGA4 is at 115 kDa.
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