Western blot of nonreduced(-) and reduced(+) mouse IgG1kappa and IgG1lambda (20ng/lane), using 0.2ug/mL of RevMAb clone RM103. This antibody reacts to nonreduced IgG1kappa (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
Western blot of nonreduced(-) and reduced(+) mouse IgG1kappa and IgG1lambda (20ng/lane), using 0.2ug/mL of RevMAb clone RM103. This antibody reacts to nonreduced IgG1kappa (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
anti-Ig Kappa Light Chain (mouse), Rabbit Monoclonal (RM103)
REV-31-1007-00
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-Ig Kappa Light Chain (mouse), Rabbit Monoclonal (RM103)
- Delivery Days Customer5
- Antibody SpecificityThis antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM103
- Concentration1 mg/ml
- FormulationLiquid
- HostRabbit
- IsotypeIgG
- Protein IDP01837
- Protein NameImmunoglobulin kappa constant
- Scientific DescriptionImmunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Kappa chains generally represent more than 90% of mouse Ig light chains. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays. - Recombinant Antibody. This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG. Applications: WB (nonreduced), IP, ICC, IHC, FACS, ELISA . Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Kappa chains generally represent more than 90% of mouse Ig light chains. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203