Anti-IgE [mAbA9]

Absolute Antibody

Anti-IgE [mAbA9]

7

This antibody is specific for the extracellular membrane-proximal domain (EMPD) of murine membrane-bound IgE. As this 19-amino acid long EMPD is isotype-specific, this antibody does not cross-react with soluble mouse IgE.

This antibody binds to the extracellular membrane-proximal domain (EMPD) of mouse membrane-bound IgE with high specificity and affinity, as confirmed in ELISA analysis and FC analysis (Feichtner et al, 2008). In mice immunised with recombinant Bet v1a, the major birch pollen allergen, subsequent passive immunization with this antibody leads to a block of newly synthesized specific IgEs, and reduces serum Bet v1a-specific IgE titers (Feichtner et al, 2008). In vitro, this antibody induces receptor-mediated apoptosis in B cells expressing membrane-bound IgE (Feichtner et al, 2008).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELISA; FC; blocking

Research Use Only

Monoclonal

mAbA9

Mouse

IgG1

Mouse

Mouse

This antibody was raised by immunising ΔM1M2 BALB/c mice, which cannot express membrane-bound IgE due to the lack of the IgE-transmembrane and IgE-cytoplasmic domains, with the extracellular membrane-proximal domain of mouse membrane IgE (EMPD) conjugated

-20°C,2°C to 8°C

12352203