Figure 1. Western blot analysis of IGFBP-1 using anti-IGFBP-1 antibody (PB9604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-1 antigen affinity purified polyclonal antibody (Catalog # PB9604) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP-1 at approximately 28 kDa. The expected band size for IGFBP-1 is at 28 kDa.
Figure 1. Western blot analysis of IGFBP-1 using anti-IGFBP-1 antibody (PB9604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-1 antigen affinity purified polyclonal antibody (Catalog # PB9604) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP-1 at approximately 28 kDa. The expected band size for IGFBP-1 is at 28 kDa.
Anti-IGFBP1 Antibody Picoband(r)
PB9604
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHamster, Mouse, Rat
TargetIgfbp1
Overview
- SupplierBoster Bio
- Product NameAnti-IGFBP-1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID16006
- Target nameIgfbp1
- Target descriptioninsulin-like growth factor binding protein 1
- Target synonymsIBP-1; IGF-binding protein 1; IGFBP; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1); insulin-like growth factor-binding protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP47876
- Protein NameInsulin-like growth factor-binding protein 1
- Scientific DescriptionBoster Bio Anti-IGFBP1 Antibody Picoband® catalog # PB9604. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203