Anti-IgG [NH3/130.5.2]

Absolute Antibody

Anti-IgG [NH3/130.5.2]

7

This antibody was shown to have a high avidity to human immunoglobulin G having a high titre, equal to a corresponding rabbit polyclonal antibody, when tested against anti-D sensitised cells (Downie et al., 1983). NH3/130.5.2 detects human IgG samples transferred from the SDS polyacrylamide gel via Western blot under non-reducing conditions (Thorpe et al., 1984). Thorne et al. (1984) mentions that this antibody was also successful in immunoprecipitation in agar or solution (M. Spitz, unpublished results). Further, the antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the antibody cross-reacted with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 as shown by ELISA analysis.

ImmunoPrecipitation, Western Blot

WB; IP

Research Use Only

Monoclonal

NH3/130.5.2

Mouse

IgG1

Human, Monkey, Primate

Human, Cynomolgus Monkey, Rhesus Macaque

This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.

-20°C,2°C to 8°C

41116161