Anti-IgG [RF61]

Absolute Antibody

Anti-IgG [RF61]

9

This antibody is specific for the C-terminal (Cgamma3) end of the Fc region of IgG1, -2, and -3, with ~10-fold lower affinity for IgG4. This antibodys binding to IgG Fc is non-inhibitable by S. aureus protein A (SpA), which binds IgG Fc at the Cgamma2-Cga

The binding activity of this antibodys original format (human IgM) to IgG Fc fragments was measured by ELISA, and a dissociation constant (Kd) of 6.0x10-7 M was calculated (Burastero et al., 1988; PMID: 3264319). This antibody exhibited strong reactivity with both monoclonal and polyclonal human anti-Rh IgG antibodies bearing the Gm(a) or Gm(g) specificity in hemagglutination assays (HAs), displaying a higher affinity for Gm(g). Additionally, it demonstrated efficient binding to heat-altered autologous Gm(a-), (g-) IgG and exhibited heteroclitic specificity at the clonal level in an autologous system (Williams Jr et al., 1992; PMID: 1380541). The binding of this antibodys original format to human IgG subclasses as measured by ELISA demonstrated that its binding specificity is IgG1~IgG2~IgG3>IgG4 (Duquerroy et al., 2007; PMID: 17395205).

ELISA, Other Application

Research Use Only

Monoclonal

RF61

Mouse

IgG1

Immunoglobulin gamma-1 heavy chain

Human

-20°C,2°C to 8°C

12352203