Anti-IgG1/2a Fab (k) [TP888]

Absolute Antibody

Anti-IgG1/2a Fab (k) [TP888]

9

This antibody recognises mouse Fab of the IgG1 and IgG2a which contain kappa light chain (the binding seems to be dependent on the CH1 region and the light chain type simultaneously).

Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, such nanobodies have been recommended as very effective for colocalisation studies when more than one fluorophore is needed (Pleiner et al., 2018).

Dot Blot

Research Use Only

Monoclonal

TP888

Mouse

IgG1

This chimeric mouse antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203