Anti-IgM [1.14]

Absolute Antibody

Anti-IgM [1.14]

9

This antibody is specific for trout IgM. It reacts with the IgM heavy chain (Hedfors et al, 2012, pmid:23009920). It is not directed against nonprotein antigenic parts of the IgM, which is shown by the failure of IgM glycopeptides to inhibit precipitation

This antibody has been used for affinity chromatography with the protein A (Deluca et al, 1983, pmid:6347695). The antibody was shown to be effective in flow cytometry application (Deluca et al, 1983, pmid:6347695). It was demonstrated that this antibody can be utilized in the immunofluorescence staining of trout head kidney, spleen and thymus cells (Deluca et al, 1983, pmid:6347695). Furthermore, it has been shown that this antibody can be used in the immunoprecepitation of trout IgM (Deluca et al, 1983, pmid:6347695). It is used in western blots as a negative against catfish IgL sigma (Edholm et al, 2010, pmid:20149930) and MHC class IIbeta (Moulana et al, 2008, pmid:18676026). Finally, 1.14 antibody can be used to detect trout antibody in an ELISA (Costa et al, 2011, pmid: 21641928).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, Other Application, Purification/Extraction/Isolation

Research Use Only

Monoclonal

1.14

Mouse

IgG1

Fish

-20°C,2°C to 8°C

12352203