Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( kappa+ lambda) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
anti-IgM (human), Rabbit Monoclonal (RM121)
REV-31-1023-00
ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-IgM (human), Rabbit Monoclonal (RM121)
- Delivery Days Customer5
- Antibody SpecificityThis antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
- ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM121
- Concentration1 mg/ml
- FormulationLiquid
- HostRabbit
- IsotypeIgG
- Protein IDP01871
- Protein NameImmunoglobulin heavy constant mu
- Scientific DescriptionImmunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In the case of humans and other mammals that have been studied, the spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. This anti-human secondary antibody has well-characterized specificity for human IgM immunoglobulins and is useful in the detection, sorting or purification of its specified target. In general, secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. - Recombinant Antibody. This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE. Applications: ICC, IHC, FACS, ELISA. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In the case of humans and other mammals that have been studied, the spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. This anti-human secondary antibody has well-characterized specificity for human IgM immunoglobulins and is useful in the detection, sorting or purification of its specified target. In general, secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203