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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Anti-IKBKE Antibody

HPA015788
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIKBKE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IKBKE
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9641
  • Target name
    IKBKE
  • Target description
    inhibitor of nuclear factor kappa B kinase subunit epsilon
  • Target synonyms
    IKK-E, IKK-i, IKKE, IKKI, inhibitor of nuclear factor kappa-B kinase subunit epsilon, I-kappa-B kinase epsilon, IKK-epsilon, IKK-related kinase epsilon, inducible I kappa-B kinase, inducible IkappaB kinase, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14164
  • Protein Name
    Inhibitor of nuclear factor kappa-B kinase subunit epsilon
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of IKKi/IKKe/IKBKE using anti-IKKi/IKKe/IKBKE antibody (A01816-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKKi/IKKe/IKBKE antigen affinity purified polyclonal antibody (Catalog # A01816-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKKi/IKKe/IKBKE at approximately 80KD. The expected band size for IKKi/IKKe/IKBKE is at 80KD.
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