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Anti-IKZF3 Antibody

Anti-IKZF3 Antibody

A99071
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-IKZF3 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to IKZF3.
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IKZF3 using anti-IKZF3 antibody (A01611-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF3 antigen affinity purified polyclonal antibody (Catalog # A01611-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKZF3 at approximately 70 kDa. The expected band size for IKZF3 is at 58 kDa.
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ReactivityHuman, Mouse, Rat
TargetIKZF3
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Product group Antibodies
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TargetIKZF3
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Product group Antibodies
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Mouse lymph node lysates probed with IKZF3 Polyclonal Antibody, Unconjugated (bs-2954R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
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ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetIKZF3
  • SizePrice
Product group Antibodies
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TargetIKZF3
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Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using Anti-IKZF3 antibody. Corresponding IKZF3 RNA-seq data are presented for the same tissues.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKZF3 antibody [N1C2] (GTX115628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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