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ProductsBack/Anti-IL-13 [DOM10-53-616]

Anti-IL-13 [DOM10-53-616]

AB03209-23.159
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetIL13
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-13 [DOM10-53-616]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding characterization of this antibody to human, cynomolgus monkey and rhesus monkey IL-13 was done using ELISA. The binding affinity of the original single chain VHH antibody was assessed using BIAcore analysis. It was reported that the original VHH antibody bound human and cynomolgus monkey IL13 with a binding affinity of 130 pM and 3.2 pM respectively. This antibody shows neutralizing potency for human, cynomolgus monkey and rhesus IL-13 (US20110236380).
  • Applications
    ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DOM10-53-616
  • Gene ID3596
  • Target name
    IL13
  • Target description
    interleukin 13
  • Target synonyms
    IL-13; interleukin-13; P600
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35225
  • Protein Name
    Interleukin-13
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL13 using anti-IL13 antibody (A00077). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL13 antigen affinity purified polyclonal antibody (Catalog # A00077) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL13 at approximately 20KD. The expected band size for IL13 is at 16KD.
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