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FACS: Human PBMCs were cultured with recombinant IL-1beta, TGF-beta, and IL-6 for 4 days. Cells were then reactivated with PMA/Ionomycin in the presence of monensin for 5h. Cells were stained with anti-IL-17A (human), mAb (17-142F) (FITC) (Prod. No. A
FACS: Human PBMCs were cultured with recombinant IL-1beta, TGF-beta, and IL-6 for 4 days. Cells were then reactivated with PMA/Ionomycin in the presence of monensin for 5h. Cells were stained with anti-IL-17A (human), mAb (17-142F) (FITC) (Prod. No. A
FACS: Human PBMCs were cultured with recombinant IL-1beta, TGF-beta, and IL-6 for 4 days. Cells were then reactivated with PMA/Ionomycin in the presence of monensin for 5h. Cells were stained with anti-IL-17A (human), mAb (17-142F) (FITC) (Prod. No. A

anti-IL-17A (human), mAb (17-142F)

AG-20A-0071
AdipoGen Life Sciences
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
TargetIL17A
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-IL-17A (human), mAb (17-142F)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    17-142F
  • Concentration
    0.5 mg/ml
  • Gene ID3605
  • Target name
    IL17A
  • Target description
    interleukin 17A
  • Target synonyms
    CTLA8; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; IL17; IL-17; IL-17A; ILA17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17; interleukin-17A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16552
  • Protein Name
    Interleukin-17A
  • Scientific Description
    IL-17A exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblsts, the enhancement of surface expression of ICAM-1 in fibroblast, activation of NF-kappaB and costimulation of T cell proliferation. Induces stromal cells to produce proinflammatory and hematopoietic cytokines. - Monoclonal Antibody. Recognizes human IL-17A. Isotype: Mouse IgG1kappa. Clone: 17-142F. Applications: ELISA, FACS. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. IL-17A exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblsts, the enhancement of surface expression of ICAM-1 in fibroblast, activation of NF-kappaB and costimulation of T cell proliferation. Induces stromal cells to produce proinflammatory and hematopoietic cytokines.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of IL17A using anti-IL17A antibody (A00421-4). IL17A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-IL17A Antibody (A00421-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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