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Anti-IL-1B [10G11]

Ab03560-1.1
Absolute Antibody
ApplicationsFunctional Assay, ELISA
Product group Antibodies
TargetIl1b
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-1B [10G11]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The original mouse IgG antibody binds mouse IL-1B with a binding affinity of Kd= 0.23 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 3.70E-10M. This antibody was used in combination with 9H10 (anti- mouse IL-1A) antibody for the generation of anti-mouse IL-1alpha/beta DVD-Ig molecule (US7612181).
  • Applications
    Functional Assay, ELISA
  • Applications Supplier
    ELISA; functional assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10G11
  • Gene ID16176
  • Target name
    Il1b
  • Target description
    interleukin 1 beta
  • Target synonyms
    IL-; IL-1 beta; Il-1b; IL-1beta; interleukin-1 beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP10749
  • Protein Name
    Interleukin-1 beta
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    The original antibody was generated by immunizing IL-1alfa/bèta double KO mice with mouse IL-1 beta.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 36 kDa. The expected band size for IL1 beta is at 31 kDa.
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