Anti-IL-1R1 [Antibody 6]

Absolute Antibody

Anti-IL-1R1 [Antibody 6]

7

This antibody binds human IL-1R1 and cynomolgus monkey IL-1R1 but does not cross react with mouse IL-1R1. This antibody binds an epitope containing amino acids in a discontinuous epitope of 33 amino acids in three regions of human IL-1R1; N123-V134, L140-

The binding affinity of this antibody to human IL-1R1 was determined using kinetic exclusion assay. It was reported that the human IgG1 version of this antibody binds human IL-1R1 with a binding affinity of 3.05 pM. The human IgG1 version of this antibody shows the inhibition of IL-1beta induced IL-8 release from HeLa cells. The IgG2 version was capable of inhibition of IL-1beta and IL-alpha induced IL-8 release from HeLa cells. The binding of this antibody was capable of inhibiting binding of IL-1Ra binding to IL-1R1. This antibody was reported to inhibit huIL-1beta-induced IL-8 release from CYNOM-K1 cells. The human IgG1 and IgG2 versions of this antibody was capable of inhibiting IL-6 production in human whole blood. The IgG2 version also blocked IL-1 activity (US8298533).

Flow Cytometry, Neutralisation/Blocking

inhibit; FC

Research Use Only

Monoclonal

Antibody 6

IL1R1

interleukin 1 receptor type 1

CD121 antigen-like family member A; CD121A; D2S1473; IL1R; IL-1R-1; IL1RA; IL-1R-alpha; IL-1RT1; IL-1RT-1; interleukin 1 receptor alpha, type I; interleukin-1 receptor alpha; interleukin-1 receptor type 1; interleukin-1 receptor type I; P80

Rabbit

IgG

Interleukin-1 receptor type 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG2 format for improved compatibility with existing reagents assays and techniques.

Human, Monkey

Human, cynomolgus monkey

The original antibody was isolated from a naïve human single chain Fv (scFv) phage display libraries using a series of selection cycles on recombinant human IL-1R1. The scFv was later reformatted to the human IgG1 version.

-20°C,2°C to 8°C

12352203