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Immunohistochemical staining of human spleen shows moderate nuclear positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate nuclear positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate nuclear positivity in cells in red pulp.

Anti-IL17B Antibody

HPA042441
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIL17B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL17B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27190
  • Target name
    IL17B
  • Target description
    interleukin 17B
  • Target synonyms
    cytokine-like protein ZCYTO7; IL-17B; IL-20; interleukin 20; interleukin-17 beta; interleukin-17B; neuronal interleukin-17 related factor; NIRF; ZCYTO7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHF5
  • Protein Name
    Interleukin-17B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
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