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Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.

Anti-IL1RL1 Antibody

HPA007406
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL1RL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL1RL1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9173
  • Target name
    IL1RL1
  • Target description
    interleukin 1 receptor like 1
  • Target synonyms
    DER4; FIT-1; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL33R; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; ST2; ST2L; ST2V; T1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01638
  • Protein Name
    Interleukin-1 receptor-like 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ST2/IL1RL1 using anti-ST2/IL1RL1 antibody (A00492-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST2/IL1RL1 antigen affinity purified polyclonal antibody (Catalog # A00492-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST2/IL1RL1 at approximately 70 kDa. The expected band size for ST2/IL1RL1 is at 63 kDa.
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Immunohistochemical staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.
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